Mass spectrometer working principle instrumentation tools. Sep 06, 2016 the first part in a series of lectures on the basics of mass spectrometry presented by lennart martens the prese. For a sample mixture, the first quadrupole mass analyzer generates a spectrum of ions. Electrospray ionization esi is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol.
Basics of mass spectrometry michal holcapek, university of pardubice, czech republic abstract mass spectrometry is used for the structural elucidation or confirmation of organic, bioorganic and organometallic compounds and for quantitative analysis in environmental, pharmaceutical, forensic, foodfeed, and other sciences. Protein analysis by mass spectrometry biomolecular. The final element of the mass spectrometer is the detector. Mass spectrometry and biotechnology resources news, tutorials, databases etc.
Tandem mass spectrometry msms, as the name indicates, is the result of performing two or more sequential separations of ions usually coupling two or more mass analyzers. When the sample passes through the electron stream between the cathode and anode, electrons with high energy knock electrons out of the sample and form ions. For more information, log on to download the study materials here. It is especially useful in producing ions from macromolecules because it overcomes the propensity of these molecules to fragment when ionized. Mass spectrometry is an aspect of science that could finally put the steroid era of baseball to an end. Muddiman north carolina state university on june, 2016. A mass spectrum looks a bit like a line graph with spikes or peaks of different heights.
Mass spectrometry basics provides authoritative yet plainspoken explanations of the basic concepts of this powerful analytical method without elaborate mathematical derivations. This video will show the concepts of tandem mass spectrometry, a general procedure, and some of its uses in biochemistry. Fourier transform mass spectrometry ftms offers advantages of high resolution and mass accuracy that are especially helpful in peptide mass fingerprinting and mass mapping. Heeren, fominstitute amolf, amsterdam, the netherlands. Youll see this written as m over z in a lot of mass spectrometry examples. So, weve seen, if you increase the mass, you increase the radius. Mass spectrometry ms has progressed to become a powerful analytical tool for both quantitative and qualitative applications. Mass spectrometry is a powerful analytical technique used to quantify known materials, to identify unknown compounds within a sample and to elucidate the structure and. In scanning mass spectrometry, one starts with a mixture of ions having dif ferent masstochar ge ratios and dif ferent relative abundances. Sciex provides the bestinclass instructorled training courses on various mass spectrometry and separation systems. Ppt mass spectrometry powerpoint presentation free to. The mass spectrometer then uses electric and magnetic fields to measure the mass weight of the charged particles. The basics of mass spectrometry in the twentyfirst century.
Mass spectrometry ms is a process that measures the mass tocharge ratio of charged particles. The advantages of mass spectrometry are high sensitivity, high mass accuracy, and more importantly, structural information. So lets get started with your introduction to mass spectroscopy. Mass spectrometry ms is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the mass tocharge ratio and abundance of gasphase ions. Hello everybody, welcome to week three of the coursera. Mass spectrometer works on mass to charge ratio principle. Tutorials, databases and tools mass spectrometry center. This article was a brief introduction to the basics of mass spectrometry, but it doesnt end there. This is the 2nd part in a series of lectures on the basics of mass spectrometry presented by lennart martens the. Basics and principle of mass spectrometry, principle of mass spectrometry, theory of mass spectrometry, molecular ions, fragment ions. Tandem mass spectrometry, usually referred to as msms, involves the use of 2 or more mass analyzers. A brief overview of a simple eimode mass spectrometry experiment. Mass spectrometry, also referred to as mass spec, is an analytical technique becoming increasingly important in bioscience research. This article will introduce you to this technique, how it works, and how it could be useful to you in your research.
An education video on mass spectrometry using a magnetic sector instrument from the royal society of chemistry. Mass spectrometry learning center thermo fisher scientific us. Mass spectrometry ms has become an integral tool in life sciences. Nested ion mobilitytof mass spectrometry presented by. Summary of the characteristics of differ ent mass analyzers. This video describes the basic principles behind mass spectrometry. Mass spectrometry request mass spectrometry services through ilab, see instructions. You can use it to find out the mass of particles, and to find the basic chemicals in a sample or molecule. The complete process involves the conversion of the sample into gaseous ions, with or without fragmentation, which are then. Educational resource from asms short tutorials on hplc, ms, gpc by waters co.
Fragmentation is virtually absent in electrospray mass spectrometry, but can be deliberately induced by msms techniques. The majority of protein sequence analysis today uses mass spectrometry. An atomic balance mass spectrometry basics is a term related to mass spectrometry. The presented teaching modules introduce young children to mass spectrometry and. This modified daltons original atomic theory because atoms of the. Mass spectrometers are used to determine which of the following. Mass spectrometry techniques department of chemistry texas. We offer easy, highthroughput, quantitative workflows for proteins, drug metabolites, pesticides and many other applications.
What may take ten minutes to analyze with another method, takes ten seconds with a mass spectrometer. Exact mass measurements remember that the mass measurement is determined for the ion and not the neutral molecule. The modules are highly adaptable and can be applied to young children from the age of 6. Mass spectrometry ms principle, working, instrumentation, steps, applications. Tandem msms employs collision induced dissociation cid to fragment a precursor ion. The authors describe processes, applications, and the underlying science in a concise manner supported by figures and graphics to further comprehension. One grid is positively charged and repels the ions. Matrix assisted laser desorptionionization maldi in maldi, there is a mixture of two different substances. The first step in ms analysis is ion formation ionization. There are various ways of producing the ion required, and the.
Mass spectrometry principle, instrumentation, working. Gas chromatography and gcms can be used to analyze a small percentage of the 9 million registered compounds. Stepup to agilent oilfree vacuum mass spectrometers. Mass spectrometry simple english wikipedia, the free. These courses blend classroom training with handson lab exercises, and cover. To know the mass spectrometer, first we have to know what is mass number mass number is also called atomic. In this section, three different mass spectrometry techniques will be discussed.
Mass spectrometry ms is an analytical chemistry technique that helps identify the amount and type of chemicals present in a sample by measuring the masstocharge ratio and abundance of gasphase. The present chapter tries to introduce the reader to the technology describing in short the basics of the instrumentation and the. This organic chemistry video tutorial provides a basic introduction into mass spectrometry. Mass spectrometry ms is an analytical laboratory technique to separate the components of a sample by their mass and electrical charge. The force required to move them comes from an electric field supplied by two metal grids. Mass spectrometry is a process that involves ionization, fragmentation, selection, detection and analysis. It can also tell you the chemical structures of molecules, such as peptides and other chemical compounds. It is used for determining masses of particles, for determining the elemental composition of a sample or molecule. Right click movies to download them if they automatically. The book begins with an overview that includes essential definitions and then provides information on the components of and the strategies used in the most common instruments. The instrument used in ms is called mass spectrometer. The presented teaching modules introduce young children to mass spectrometry and give them the opportunity to experience a modern research laboratory. The mass spectrometer has a fast and linear response. Mass spectrometry is a powerful tool for the characterization of various biomolecules including nucleosides, nucleotides, oligonucleotides, and nucleic acids.
In laboratory research, it is important to understand these techniques. Mass spectrometry is a powerful technique with many different applications in biology, chemistry, and physics, but also in clinical medicine and even in space exploration. Mass spectrometry principles and applications 3rd edition. The four main parts of mass spectrometry are discussed below. The actual device used to carry out mass spectrometry. The first mass spectrometer was constructed in 1912 and. The mass analyzer separates the ions according to their mass to charge ratio mz. It is often used to analyze individual components in a mixture. Basics mass spectrometry facility university of colorado.
Mass spectrometry is an analytical technique to measure the masstocharge ratio of ions that can be used to identify unknown compounds, determine the isotopic composition of. Development of two ionization techniques, electrospray esi and matrix assisted laser. This paper covers the basics of mass spectrometry instrumentation and introduces the interpretation of mass spectra. This short video explains the basics of the quadrupole mass spectrometer. Electrospray, maldi, and commonly used mass analyzers. Lecture mass spectrometry basics part 2 of 7 youtube. New omics techniques and especially peptidomics, based on mass spectrometry ms, have been applied to peptide structure elucidation. The biomolecular analysis facility core has been awarded funding from the virginia equipment trust fund. Next, the positive ions must move out of the ionization chamber. Mass spectrometry for the novice provides exactly that, with detailed, straightforward descriptions and clear illustrations of principles of operations and techniques. For our purposes, were just trying to think about how the mass affects the radius of the circle that the ion will move in. Introduction mass spectrometry ms is an analytical technique that measures the masstocharge particles. Mass spectrometry can be used to determine the accurate molecular mass and structural features of an organic compound. Many mass spectrometers use two or more mass analyzers for tandem mass spectrometry msms.
Mass spectrometry ms is an analytical chemistry technique that helps identify the amount and type of. Because introduction liquid chromatography mass spectrometry lcms is fast becoming the preferred tool of liquid chromatographers. Typical mass spectrometry research focuses on the formation of gas phase ions, the chemistry of ions, and applications of mass spectrometry. Mass spectrometry tutorial chemical instrumentation. Mass spectrometry an overview sciencedirect topics. The number of hits may also be reduced by taking an accurately measured isotope pattern of the ion into consideration. Aug 08, 20 009 mass spectrometry in this video paul andersen explains how a spectrometer was used to identify the presence of isotopes. Thermo scientific high resolution mass spectrometry systems take discovery and sample analysis to new heights. Tandem mass spectrometry selects a molecule of interest that is later fragmented into multiple subunits, which can help elucidate its identification and sequence. Mass spectrometry basics is a pedagogy for novice practitioners and those with little or no experience in the field of mass spectrometry. A short explanation for the absolute novice what does a mass spectrometer do. Mass spectrometry is a powerful analytical technique used to quantify known materials, to identify unknown compounds within a sample, and to elucidate the structure and chemical properties of different molecules.
How do all these steps come together to elucidate the amounts and chemical identities of target analytes. Basics of mass spectrometry basics of mass spectrometry. The authors describe processes, applications, and the underlying science in a concise manner supported by figures and gr. Either way, this first step of mass spectrometry produces positive ions.
A mass spectrometer produces charged particles ions from the chemical substances that are to be analyzed. Whether youre detecting disease biomarkers or performing forensic analysis on drugs of abuse, our mass. Fragmentation takes place producing parent ion and ion. Both quadrupole and time of flight tof mass analyzers are commonly used and they can be configured together as qtof tandem mass spectrometric instruments. A mass spectrum plural spectra is a plot of the ion signal as a function of the mass tocharge ratio. Fundamentals and applications of fourier transform mass. It includes information on ionisation processes, techniques, instruments, and general ms and combined ms applications. Mass spectrometry ms is commonly regarded as an instrumental technique for separation of electrically charged species in the gas phase for general information, see for example.
Mass spectrometry, also called mass spectroscopy, analytic technique by which chemical substances are identified by the sorting of gaseous ions in electric and magnetic fields according to. This short flash animation video outlines the basic principles of a mass spectrometer. Mass spectrometry has become an indispensable analytical tool in all types of drug discovery applications. It is used by separating molecular ions on the basis of their mass and charge to determine the molecular weight of compounds. Introduction mass spectrometry ms is an analytical technique that measures the mass tocharge particles. Download these resources for yourself or to share with others. Hanan awad college of pharmacy and nutrition, university of saskatchewan, saskatoon.380 1344 1011 35 183 794 1580 1479 1404 978 145 391 1147 1550 1028 988 853 1265 30 505 1011 202 1129 423 218 1241 154 1364 1474 240 794 468 960 809